Article

Single-cell Characterization of DNA Hydroxymethylation of the Mouse Brain During Aging

2025-06-01

Abstract excerpt

DNA methylation dynamics, including 5-hydroxymethylcytosine (5hmC) and 5-methylcytosine (5mC), critically regulate brain function, yet conventional methods cannot distinguish these modifications, obscuring their distinct roles in gene regulation and aging. We present Joint- Cabernet, a bisulfite-free single-cell platform enabling simultaneous profiling of 5hmC, 5mC, and transcriptomes. Applying Joint-Cabernet to 8...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: cd2f0be2-82ca-52ff-b09e-e22de50a7694
DOI: 10.1101/2025.05.29.656780

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Single-cell Characterization of DNA Hydroxymethylation of the Mouse Brain During AgingDOI 10.1101/2025.05.29.656780Read

An Integrative Single-Cell Epigenomic Atlas Annotates the Regulatory Genome of the Adult Mouse BrainSemantic proximity Read

Cell-type-specific transposable element demethylation and TAD remodeling in the aging mouse brainSemantic proximity Read

Differential usage of DNA modifications in neurons, astrocytes, and microgliaSemantic proximity Read

Epigenomic complexity of the human brain revealed by single-cell DNA methylomes and 3D genome structuresSemantic proximity Read

Postnatal conversion of methylcytosine to hydroxymethylcytosine reconfigures the human neuronal epigenomeSemantic proximity Read

Whole-genome profiling of native 5-hydroxymethylation in human neurons with long-read sequencingSemantic proximity Read

Gene body DNA hydroxymethylation restricts the magnitude of transcriptional changes during agingSemantic proximity Read

A theoretical and experimental framework enables low-coverage sequencing for accurate quantification of genome-wide cytosine modification levelsSemantic proximity Read

Early microglial priming in Alzheimer’s disease revealed by ME-seqSemantic proximity Read

A global view of aging and Alzheimer’s pathogenesis-associated cell population dynamics and molecular signatures in the human and mouse brainsSemantic proximity Read

An epigenome atlas of neural progenitors within the embryonic mouse forebrainSemantic proximity Read

Human Body Single-Cell Atlas of 3D Genome Organization and DNA MethylationSemantic proximity Read