Article

Comparative characterization of Cas12a2 orthologs identifies high-activity nucleases for programmable cell elimination

2026-06-24

Abstract excerpt

Cas12a2 CRISPR nucleases, including SuCas12a2, have been shown to have extensive collateral activity towards RNA, ssDNA, and dsDNA. This collateral activity results in targeted cell elimination and has applications across biotechnology, agriculture, and human health. We explored the natural genetic diversity of Cas12a2 nucleases and characterized nine novel orthologs in a DNA damage kinetic assay in E. coli. Three...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 73da2612-a963-518b-845a-793312ee9e40
DOI: 10.64898/2026.06.23.734040

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Comparative characterization of Cas12a2 orthologs identifies high-activity nucleases for programmable cell eliminationDOI 10.64898/2026.06.23.734040Read

Cas12e orthologs evolve variable structural elements to facilitate dsDNA cleavageSemantic proximity Read

CRISPR-Cas12a REC2 – NUC interactions drive target-strand cleavage and constrain trans cleavageSemantic proximity Read

Harnessing Non-Canonical crRNAs to Improve Functionality of Cas12a OrthologsSemantic proximity Read

A Combinatorial Approach towards Adaptability of 22 Functional Cas12a Orthologs for Nucleic Acid Detection in Clinical SamplesSemantic proximity Read

Nuc domain electrostatics drive the trans cleavage activity of CRISPR-Cas12a.Semantic proximity Read

Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs.Semantic proximity Read

Massively parallel kinetic profiling of natural and engineered CRISPR nucleasesSemantic proximity Read

Harnessing noncanonical trans-cleavage characteristics of Cas12 and Cas13a to enhance CRISPR-based diagnosticsSemantic proximity Read

μCas, a novel class of miniature type-V Cas12f nucleases with diverse PAMSemantic proximity Read

PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavageSemantic proximity Read

Characterization of diverse Cas9 orthologs for genome and epigenome editingSemantic proximity Read

Fusion of a non-specific DNA-binding domain enhances Cas12a trans-cleavage for robust nucleic-acid diagnosticsSemantic proximity Read