Engineering Highly Thermostable Cas12b via De Novo Structural Analyses for One-Pot Detection of Nucleic Acids

Abstract excerpt

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based diagnostics have elevated nucleic acid detection in terms of sensitivity, specificity, and rapidity in recent years. CRISPR-Cas systems can be combined with a pre-amplification step in a one-pot reaction to simplify workflow and reduce carryover contamination. Here, we report an engineered Cas12b system from Brevibacillus (eBrCas12b) with imp...

Topics

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• Biosensing Techniques
• COVID-19
• CRISPR and Genetic Engineering
• CRISPR-Cas Systems
• Hepatitis B virus
• Molecular Diagnostic Techniques
• Nucleic Acid Amplification Techniques
• SARS-CoV-2

Identifiers and source

Literature Corpus work

c2fd8eca-7475-56c5-8943-0ffb45ee614c

DOI

10.1101/2022.10.02.22280626