Article

Chimeric CRISPR guides enhance Cas9 target specificity

2017-06-08

Abstract excerpt

Oligonucleotide-guided nucleases (OGNs) have enabled rapid advances in precision genome engineering. Though much effort has gone into characterizing and mitigating mismatch tolerance for the most widely adopted OGN, Streptococcus pyogenes Cas9 (SpCas9), potential off-target interactions may still limit applications where on-target specificity is critical. Here we present a new axis to control mismatch sensitivity...

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Literature Corpus work: c79ffe0c-e480-5c61-afde-d0ce8670a7e7
DOI: 10.1101/147686

Open publication

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