Article
Profiling single-guide RNA specificity reveals a mismatch sensitive core sequence.
Scientific reports - 18 Jan 2017
Zheng Ting, Hou Yingzi, Zhang Pingjing, Zhang Zhenxi, Xu Ying, Zhang Letian, Niu Leilei, Yang Yi, Liang Da, Yi Fan, Peng Wei, Feng Wenjian, Yang Ying, Chen Jianxin, Zhu York Yuanyuan, Zhang Li-He, Du Quan
Abstract excerpt
Targeting specificity is an essential issue in the development of CRISPR-Cas technology. Using a luciferase activation assay, off-target cleavage activity of sgRNA was systematically investigated on single nucleotide-mismatched targets. In addition to confirming that PAM-proximal mismatches are less tolerated than PAM-distal mismatches, our study further identified a "core" sequence that is highly sensitive to...
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