Article
Partial DNA-guided Cas9 enables genome editing with reduced off-target activity.
Nature chemical biology - 1 Mar 2018
Yin Hao, Song Chun-Qing, Suresh Sneha, Kwan Suet-Yan, Wu Qiongqiong, Walsh Stephen, Ding Junmei, Bogorad Roman L, Zhu Lihua Julie, Wolfe Scot A, Koteliansky Victor, Xue Wen, Langer Robert, Anderson Daniel G
Abstract excerpt
CRISPR-Cas9 is a versatile RNA-guided genome editing tool. Here we demonstrate that partial replacement of RNA nucleotides with DNA nucleotides in CRISPR RNA (crRNA) enables efficient gene editing in human cells. This strategy of partial DNA replacement retains on-target activity when used with both crRNA and sgRNA, as well as with multiple guide sequences. Partial DNA replacement also works for crRNA of Cpf1,...
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