Article

CRISPR-Cas9 knockout screen informs efficient reduction of the <i>Komagataella phaffii</i> secretome

2024-04-21

Abstract excerpt

The yeast Komagataella phaffii is widely used for manufacturing recombinant proteins, but secreted titers of recombinant proteins could be improved by genetic engineering. In this study, we hypothesized that cellular resources could be redirected from production of endogenous proteins to production of recombinant proteins be deletion of unneeded endogenous proteins. We identified a set of endogenous secreted prot...

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Literature Corpus work: c49f8bf5-cf1a-55d9-b02d-eb8074b62f95
DOI: 10.1101/2024.04.21.590449

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