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Article

An inducible CRISPRi system for phenotypic analysis of essential genes in Pseudomonas aeruginosa.

mBio - 11 Mar 2026

Sullivan Jaryd R, Ferrara Kristina M, Barrick Rebecca, Romano Keith P, Warrier Thulasi, Hung Deborah T

Abstract excerpt

Precise and tunable genetic tools are essential for high-throughput functional genomics. To address this need in the important gram-negative pathogen Pseudomonas aeruginosa, we developed and characterized a tightly regulated CRISPR-interference (CRISPRi) system that enables precise and tunable repression of essential genes. The system utilizes a rhamnose-inducible promoter to control both the Streptococcus...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 41670347
DOI: 10.1128/mbio.02767-25
PMCID: PMC12977507

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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