Article

Identification of Essential Protein Domains From High-density Transposon Insertion Sequencing

2021-06-17

Abstract excerpt

A first clue to gene function can be obtained by examining whether a gene is required for life in certain standard conditions, that is, whether a gene is essential. In bacteria, essential genes are usually identified by high-density transposon mutagenesis followed by sequencing of insertion sites (Tn-seq). These studies assign the term “essential” to whole genes rather than the protein domain sequences that confer...

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Literature Corpus work: 3d95cf3b-6352-5c23-92dc-76be62b552bb
DOI: 10.21203/rs.3.rs-589027/v1

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