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Article

Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in Komagataella phaffii.

ACS synthetic biology - 17 Jan 2020

Dalvie Neil C, Leal Justin, Whittaker Charles A, Yang Yuchen, Brady Joseph R, Love Kerry R, Love J Christopher

Abstract excerpt

There is growing interest in the use of nonmodel microorganisms as hosts for biopharmaceutical manufacturing. These hosts require genomic engineering to meet clinically relevant product qualities and titers, but the adaptation of tools for editing genomes, such as CRISPR-Cas9, has been slow for poorly characterized hosts. Specifically, a lack of biochemical characterization of RNA polymerase III transcription has...

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Identifiers

PMID: 31825599
DOI: 10.1021/acssynbio.9b00372
PMCID: PMC7814401

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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