Article

Identification of the toxic 6mer seed consensus in human cancer cells

2020-12-22

Abstract excerpt

6mer seed toxicity is a novel anti-cancer mechanism that kills cancer cells by triggering death induced by survival gene elimination (DISE). It is based on si- or shRNAs with a specific G-rich nucleotide composition in position 2-7 of their guide strand. An arrayed screen of 4096 6mer seeds on two human and two mouse cell lines identified a consensus GGGGGC as the most toxic seed. After testing two more cell lines...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: c43fb6f6-cd69-53e2-923d-16236982746b
DOI: 10.1101/2020.12.22.424040

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Identification of the toxic 6mer seed consensus in human cancer cellsDOI 10.1101/2020.12.22.424040Read

SPOROS: A pipeline to analyze DISE/6mer seed toxicitySemantic proximity Read

Experimental validation of the importance of seed complement frequency to siRNA specificity.Semantic proximity Read

6mer seed toxicity in viral microRNAsSemantic proximity Read

The balance between toxic versus nontoxic microRNAs determines platinum sensitivity in ovarian cancerSemantic proximity Read

A high-content screen profiles cytotoxic microRNAs in pediatric and adult glioblastoma cells and identifies miR-1300 as a potent inducer of cytokinesis failureSemantic proximity Read

Exploring genetic interactions with G-quadruplex structuresSemantic proximity Read

Small interfering RNAs based on huntingtin trinucleotide repeats are highly toxic to cancer cellsSemantic proximity Read

Death Induced by Survival gene Elimination (DISE) is correlated with neurotoxicity in Alzheimer’s disease and agingSemantic proximity Read

Expanded gene targeting in RNA hacking with G-tract-supply Staple oligomerSemantic proximity Read

A gapmer antisense oligonucleotide targeting SRRM4 is a novel therapeutic medicine for lung cancerSemantic proximity Read

Targeting oncogenic microRNAs from the miR-371~373 and miR-302/367 clusters in malignant germ cell tumours causes growth inhibition through cell cycle disruptionSemantic proximity Read

Toward optimization of AgoshRNA molecules that use a non-canonical RNAi pathway: variations in the top and bottom base pairs.Semantic proximity Read