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Article

Experimental validation of the importance of seed complement frequency to siRNA specificity.

RNA (New York, N.Y.) - 1 May 2008

Anderson Emily M, Birmingham Amanda, Baskerville Scott, Reynolds Angela, Maksimova Elena, Leake Devin, Fedorov Yuriy, Karpilow Jon, Khvorova Anastasia

Abstract excerpt

Pairing between the hexamer seed region of a small interfering RNA (siRNA) guide strand (nucleotides 2-7) and complementary sequences in the 3' UTR of mature transcripts has been implicated as an important element in off-target gene regulation and false positive phenotypes. To better understand the association between seed sequences and off-target profiles we performed an analysis of all possible (4096) hexamers...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 18367722
DOI: 10.1261/rna.704708
PMCID: PMC2327361

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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