Article

<i>In vivo</i> seamless genetic engineering via CRISPR-triggered single-strand annealing

2022-06-18

Abstract excerpt

Precise genome engineering is essential for both basic and applied research. CRISPR/Cas accelerated the speed and ease by which defined exogenous sequences are integrated into specific loci. Nevertheless, knock-in generation in multicellular animals remains challenging, partially due to the complexity of insertion screening. Even when achieved, the analysis of protein localization can still be unfeasible in highly...

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Literature Corpus work: c2ec4998-0435-5e4e-8bc4-8a79c2629ff0
DOI: 10.1101/2022.06.17.496589

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