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Efficient genome editing in primary cells and in vivo using viral-derived “Nanoblades” loaded with Cas9/sgRNA ribonucleoproteins

2017-10-12

Abstract excerpt

Programmable nucleases have enabled rapid and accessible genome engineering in eukaryotic cells and living organisms. However, their delivery into target cells can be technically challenging when working with primary cells or in vivo . Using engineered murine leukemia virus-like particles loaded with Cas9/sgRNA ribonucleoproteins (“Nanoblades”), we were able to induce efficient genome-editing in cell lines and pr...

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Literature Corpus work: 8bd5f47b-ffb8-5d0b-a9ab-9016e6a0a8b3
DOI: 10.1101/202010

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Efficient genome editing in primary cells and <i>in vivo</i> using viral-derived “Nanoblades” loaded with Cas9/sgRNA ribonucleoproteins

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