Rapid, modular, and cost-effective generation of donor DNA constructs for CRISPR-based gene knock-in

Abstract excerpt

<h4>ABSTRACT</h4> CRISPR-based gene knock-in at endogenous sites is desirable in multiple fields such as quantitative studies of signal transduction pathways and gene regulation, synthetic biology, and disease modeling. Contrasting the knock-out procedure, a key step of CRISPR knock-in procedure relies on the homology-directed repairing (HDR) process that requires a donor construct as repair template. Therefore,...

Topics

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• Cell Line
• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR-Cas Systems
• DNA
• Gene Editing
• Mutation
• RNA Editing
• RNA, Guide, CRISPR-Cas Systems

Identifiers and source

Literature Corpus work

46ef3aaa-d39c-518c-ae5f-bc494a0f5096

DOI

10.1101/219618