Article

Multiplexed sgRNA Expression Allows Versatile Single Non-repetitive DNA Labeling and Endogenous Gene Regulation

2017-03-29

Abstract excerpt

<h4>ABSTRACT</h4> The CRISPR/Cas9 system has made significant contribution to genome editing, gene regulation and chromatin studies in recent years. High-throughput and systematic investigations into the multiplexed biological systems and disease conditions require simultaneous expression and coordinated functioning of multiple sgRNAs. However, current co-transfection based sgRNA co-expression systems remain poor...

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Literature Corpus work: e985e08e-6512-59a1-82a6-b201ab2b7953
DOI: 10.1101/121905

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