Article

CRISPRi-seq in <i>Haemophilus influenzae</i> reveals genome-wide and medium-specific growth determinants

2025-08-07

Abstract excerpt

Work in the human pathobiont Haemophilus influenzae has pioneered functional genomics in bacteria such as genome-wide transposon mutagenesis combined with deep sequencing. These approaches unveiled a large set of likely essential genes, but functional studies are hampered due to a limited molecular toolbox. To bridge this gap, we engineered a titratable anhydrotetracycline (aTc)-inducible CRISPRi (Clustered Regul...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: aeeb8596-abe9-5d97-aa8d-e40e2750e5bd
DOI: 10.1101/2025.08.05.668841

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

CRISPRi-seq in <i>Haemophilus influenzae</i> reveals genome-wide and medium-specific growth determinantsDOI 10.1101/2025.08.05.668841Read

Exploration of bacterial bottlenecks and <i>Streptococcus pneumoniae</i> pathogenesis by CRISPRi-seqSemantic proximity Read

Strain-resolved CRISPRi-Seq reveals conserved antibiotic vulnerabilities in <i>Staphylococcus aureus</i>Semantic proximity Read

Mobile-CRISPRi: Enabling Genetic Analysis of Diverse BacteriaSemantic proximity Read

The impact of genetic diversity on gene essentiality within the Escherichia coli species.Semantic proximity Read

A CRISPRi-based genetic resource to study essential <i>Staphylococcus aureus</i> genesSemantic proximity Read

Comparative Tn-Seq reveals common daptomycin resistance determinants in <i>Staphylococcus aureus</i> despite strain-dependent differences in essentiality of shared cell envelope genesSemantic proximity Read

A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae.Semantic proximity Read

Multiplex CRISPRi-Cas9 silencing of planktonic and stage-specific biofilm genes in <i>Enterococcus faecalis</i>Semantic proximity Read

High-throughput CRISPRi phenotyping in <i>Streptococcus pneumoniae</i> identifies new essential genes involved in cell wall synthesis and competence developmentSemantic proximity Read

Pangenome evaluation of gene essentiality in <i>Streptococcus pyogenes</i>Semantic proximity Read

An efficient <i>in vivo</i> -inducible CRISPR interference system for group A <i>Streptococcus</i> genetic analysis and pathogenesis studiesSemantic proximity Read

Fitness determinants of vancomycin-resistant <i>Enterococcus faecium</i> during growth in human serumSemantic proximity Read