Article

A CRISPRi-based genetic resource to study essential <i>Staphylococcus aureus</i> genes

2022-11-01

Abstract excerpt

We have optimized a CRISPR interference system to facilitate gene knockdown in the gram-positive bacterial pathogen Staphylococcus aureus. Our approach used a CRISPRi system derived from Streptococcus pyogenes, which involves the co-expression of the dcas9 gene encoding a catalytically inactive Cas9 protein and a customizable single guide RNA (sgRNA). In our system, dcas9 is expressed from a single copy in the...

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Literature Corpus work: 96053bd9-a705-5d6d-be81-3715d5cd53e0
DOI: 10.1101/2022.10.31.514627

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