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Catalytic rewiring of RuvC-II catalytic site activates <i>trans</i> -cleavage in Fanzor2

2026-06-24

Abstract excerpt

Fanzors are eukaryotic RNA-guided endonucleases that mediate programmable cis -cleavage in eukaryotic cells, but their potential target-induced collateral ( trans ) cleavage activity remains largely unexplored. Here, we show that catalytic rewiring of the RuvC-II catalytic site activates robust trans -cleavage activity in Acanthamoeba polyphaga mimivirus Fanzor2 (ApmFz2). The representative variant ApmFz2-EP d...

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Identifiers and source

Literature Corpus work
b49f58ad-d747-5113-9cd8-f1f7eda36ec1
DOI
10.64898/2026.06.23.733943
Open publication

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Catalytic rewiring of RuvC-II catalytic site activates <i>trans</i> -cleavage in Fanzor2DOI 10.64898/2026.06.23.733943
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