Article
Engineered Cpf1 variants with altered PAM specificities.
Nature biotechnology - 1 Aug 2017
Gao Linyi, Cox David B T, Yan Winston X, Manteiga John C, Schneider Martin W, Yamano Takashi, Nishimasu Hiroshi, Nureki Osamu, Crosetto Nicola, Zhang Feng
Abstract excerpt
The RNA-guided endonuclease Cpf1 is a promising tool for genome editing in eukaryotic cells. However, the utility of the commonly used Acidaminococcus sp. BV3L6 Cpf1 (AsCpf1) and Lachnospiraceae bacterium ND2006 Cpf1 (LbCpf1) is limited by their requirement of a TTTV protospacer adjacent motif (PAM) in the DNA substrate. To address this limitation, we performed a structure-guided mutagenesis screen to increase...
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