Article

Engineered Cpf1 Enzymes with Altered PAM Specificities

2016-12-04

Abstract excerpt

<h4>Summary</h4> The RNA-guided endonuclease Cpf1 is a promising tool for genome editing in eukaryotic cells 1-5 . Compared to other genome editing platforms, Cpf1 offers distinct advantages, such as the ability to easily target multiple genes simultaneously 3 , as well as low rates of off-target activity 4, 5 . However, the Acidaminococcus sp. BV3L6 Cpf1 (AsCpf1), which has been successfully harnessed for ge...

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Literature Corpus work: 58ad2470-0ffa-522f-8a79-1a620dbfaba0
DOI: 10.1101/091611

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