Article

Efficient installation of heterozygous mutations in human pluripotent stem cells using prime editing

2025-05-26

Abstract excerpt

<h4>SUMMARY</h4> The utility of human pluripotent stem cells (hPSCs) is greatly enhanced by the ability to introduce precise, site-specific genetic modifications with minimal off-target effects. Although Cas9 endonuclease is an exceptionally efficient gene-editing tool, its propensity for generating biallelic modifications often limits its capacity for introducing heterozygous variants. Here, we use prime editing...

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Literature Corpus work: 299578e6-3b42-5cb3-ba59-661c5fe2cf7a
DOI: 10.1101/2025.05.25.656043

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