TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single- and Multi-Locus Genome Engineering

Abstract excerpt

<title>Abstract</title> <p>Prime editing (PE) holds immense promise for precise genome editing, but its wider adoption is constrained by large payloads and inefficient delivery in recalcitrant cell types. Here, we present TRU-PE (Trackable, Robust, and Universal Prime Editor), a toolkit that overcomes these barriers via a split-vector architecture coupled with fluorescence-guided enrichment. This design ensures s...

Topics

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• Amyloid Neuropathies, Familial
• CRISPR and Genetic Engineering
• CRISPR-Cas Systems
• Gene Editing
• Genome
• Mutation
• RNA, Guide, CRISPR-Cas Systems
• Virus-based gene therapy research

Identifiers and source

Literature Corpus work

9f1a55d7-f444-5ca4-aaf4-5f21213ec1c5

DOI

10.21203/rs.3.rs-7705396/v1