Article

Gene editing in rat embryonic stem cells to produce <i>in vitro</i> models and <i>in vivo</i> reporters

2017-02-27

Abstract excerpt

<h4>Summary</h4> Rat embryonic stem (ES) cells offer the potential for sophisticated genome engineering in this valuable biomedical model species. However, germline transmission has been rare following conventional homologous recombination and clonal selection. Here we used the CRISPR/Cas9 system to target genomic mutations and insertions. We first evaluated utility for directed mutagenesis and recovered clones w...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: b06e156c-d306-5bc1-93be-f63d188c541c
DOI: 10.1101/112276

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Gene editing in rat embryonic stem cells to produce <i>in vitro</i> models and <i>in vivo</i> reportersDOI 10.1101/112276Read

Gene Editing in Rat Embryonic Stem Cells to Produce In Vitro Models and In Vivo Reporters.Semantic proximity Read

Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation.Semantic proximity Read

i-GONAD (improved genome-editing via oviductal nucleic acids delivery), a convenient in vivo tool to produce genome-edited rats.Semantic proximity Read

Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells.Semantic proximity Read

Genetic manipulations in the rat: progress and prospects.Semantic proximity Read

Genetic modification and screening in rat using haploid embryonic stem cells.Semantic proximity Read

CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos.Semantic proximity Read

CRISPR activation rescues abnormalities in <i>SCN2A</i> haploinsufficiency-associated autism spectrum disorderSemantic proximity Read

<i>TAF1</i> -gene editing impairs Purkinje cell morphology and functionSemantic proximity Read

Generation of site-specific mutations in the rat genome via CRISPR/Cas9.Semantic proximity Read

<i>In vivo</i> seamless genetic engineering via CRISPR-triggered single-strand annealingSemantic proximity Read

Efficient and rapid generation of large genomic variants in rats and mice using CRISMERESemantic proximity Read