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Article

CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos.

Nature protocols - 1 Oct 2014

Shao Yanjiao, Guan Yuting, Wang Liren, Qiu Zhongwei, Liu Meizhen, Chen Yuting, Wu Lijuan, Li Yongmei, Ma Xueyun, Liu Mingyao, Li Dali

Abstract excerpt

Conventional embryonic stem cell (ESC)-based gene targeting, zinc-finger nuclease (ZFN) and transcription activator-like effector nuclease (TALEN) technologies are powerful strategies for the generation of genetically modified animals. Recently, the CRISPR/Cas system has emerged as an efficient and convenient alternative to these approaches. We have used the CRISPR/Cas system to generate rat strains that carry...

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PMID: 25255092
DOI: 10.1038/nprot.2014.171

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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