Article
CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos.
Nature protocols - 1 Oct 2014
Shao Yanjiao, Guan Yuting, Wang Liren, Qiu Zhongwei, Liu Meizhen, Chen Yuting, Wu Lijuan, Li Yongmei, Ma Xueyun, Liu Mingyao, Li Dali
Abstract excerpt
Conventional embryonic stem cell (ESC)-based gene targeting, zinc-finger nuclease (ZFN) and transcription activator-like effector nuclease (TALEN) technologies are powerful strategies for the generation of genetically modified animals. Recently, the CRISPR/Cas system has emerged as an efficient a...
Topics
- Animals
- Clustered Regularly Interspaced Short Palindromic Repeats
- Embryo, Mammalian
- Gene Knock-In Techniques
- Gene Knockout Techniques
- Gene Targeting
- Genome
- Homologous Recombination
- Microinjections
- Mutation
- Rats, Sprague-Dawley
- Rats, Transgenic