Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation.

Scientific reports - 29 Nov 2017

Remy Séverine, Chenouard Vanessa, Tesson Laurent, Usal Claire, Ménoret Séverine, Brusselle Lucas, Heslan Jean-Marie, Nguyen Tuan Huan, Bellien Jeremy, Merot Jean, De Cian Anne, Giovannangeli Carine, Concordet Jean-Paul, Anegon Ignacio

Abstract excerpt

The generation of gene-edited animals using the CRISPRs/Cas9 system is based on microinjection into zygotes which is inefficient, time consuming and demands high technical skills. We report the optimization of an electroporation method for intact rat zygotes using sgRNAs and Cas9 protein in combination or not with ssODNs (~100 nt). This resulted in high frequency of knockouts, between 15 and 50% of analyzed...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Clustered Regularly Interspaced Short Palindromic Repeats
Share to CRISPR-Associated Protein 9

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Electroporation

Share to Genotype

Share to Microscopy, Confocal

Share to Mutation

Share to Zygote

Source

Open in PubMed Open full text

Identifiers

PMID: 29185448
DOI: 10.1038/s41598-017-16328-y
PMCID: PMC5707420

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation.PMID 29185448Read ↗

i-GONAD (improved genome-editing via oviductal nucleic acids delivery), a convenient in vivo tool to produce genome-edited rats.Semantic proximity Read ↗

Electroporation and genetic supply of Cas9 increase the generation efficiency of CRISPR/Cas9 knock-in alleles in C57BL/6J mouse zygotes.Semantic proximity Read ↗

Generation of site-specific mutations in the rat genome via CRISPR/Cas9.Semantic proximity Read ↗

CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos.Semantic proximity Read ↗

Gene Editing in Rat Embryonic Stem Cells to Produce In Vitro Models and In Vivo Reporters.Semantic proximity Read ↗

Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes.Semantic proximity Read ↗

Successful production of genome-edited rats by the rGONAD method.Semantic proximity Read ↗

Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases.Semantic proximity Read ↗

Gene editing in rat embryonic stem cells to produce <i>in vitro</i> models and <i>in vivo</i> reportersSemantic proximity Read ↗

Optimization of the production of knock-in alleles by CRISPR/Cas9 microinjection into the mouse zygote.Semantic proximity Read ↗

Combi-CRISPR: combination of NHEJ and HDR provides efficient and precise plasmid-based knock-ins in mice and rats.Semantic proximity Read ↗

Genetic manipulations in the rat: progress and prospects.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.