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Article

Gene Editing in Rat Embryonic Stem Cells to Produce In Vitro Models and In Vivo Reporters.

Stem cell reports - 10 Oct 2017

Chen Yaoyao, Spitzer Sonia, Agathou Sylvia, Karadottir Ragnhildur Thora, Smith Austin

Abstract excerpt

Rat embryonic stem cells (ESCs) offer the potential for sophisticated genome engineering in this valuable biomedical model species. However, germline transmission has been rare following conventional homologous recombination and clonal selection. Here, we used the CRISPR/Cas9 system to target genomic mutations and insertions. We first evaluated utility for directed mutagenesis and recovered clones with biallelic...

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Identifiers

PMID: 29020614
DOI: 10.1016/j.stemcr.2017.09.005
PMCID: PMC5639479

Genes

SOX10

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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