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Article

i-GONAD (improved genome-editing via oviductal nucleic acids delivery), a convenient in vivo tool to produce genome-edited rats.

Scientific reports - 13 Aug 2018

Takabayashi Shuji, Aoshima Takuya, Kabashima Katsuya, Aoto Kazushi, Ohtsuka Masato, Sato Masahiro

Abstract excerpt

Zygote-microinjection or in vitro electroporation of isolated zygotes are now widely used methods to produce genome-edited mice. However, these technologies require laborious and time-consuming ex vivo handling of fertilized eggs, including zygote isolation, gene delivery into zygotes and embryo transfer into recipients. We recently developed an alternative method called improved genome-editing via oviductal...

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Identifiers

PMID: 30104681
DOI: 10.1038/s41598-018-30137-x
PMCID: PMC6089882

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