Article

Strain-resolved CRISPRi-Seq reveals conserved antibiotic vulnerabilities in <i>Staphylococcus aureus</i>

2025-10-13

Abstract excerpt

Staphylococcus aureus remains a major clinical threat due to rising antibiotic resistance and high rates of treatment failure. Deciphering the genetic responses to antibiotic pressure and identifying conserved vulnerabilities are essential steps toward developing broadly effective therapies. Here, we constructed strain-resolved CRISPR interference (CRISPRi) libraries targeting all genes in four clinically relevant...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 8a0924de-dad0-5241-b0bc-b3504f25ccdf
DOI: 10.1101/2025.10.13.682098

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Strain-resolved CRISPRi-Seq reveals conserved antibiotic vulnerabilities in <i>Staphylococcus aureus</i>DOI 10.1101/2025.10.13.682098Read

Genetic context drives evolution of divergent antibiotic survival phenotypes in <i>Staphylococcus epidermidis</i>Semantic proximity Read

Comparative Tn-Seq reveals common daptomycin resistance determinants in <i>Staphylococcus aureus</i> despite strain-dependent differences in essentiality of shared cell envelope genesSemantic proximity Read

Multi-strain Tn-Seq reveals common daptomycin resistance determinants in Staphylococcus aureusSemantic proximity Read

A functional genetic landscape of antibiotic sensitivity across the pneumococcal pangenome reveals conserved and lineage-specific vulnerabilitiesSemantic proximity Read

Precision Genome Editing Unveils a Breakthrough in Reversing Antibiotic Resistance: CRISPR/Cas9 Targeting of Multi-Drug Resistance Genes in Methicillin-Resistant <i>Staphylococcus aureus</i>Semantic proximity Read

A high-throughput cytotoxicity screening platform reveals <i>agr</i> -independent mutations in bacteraemia-associated <i>Staphylococcus aureus</i> that promote intracellular persistenceSemantic proximity Read

A mechanistic understanding of the effect of <i>Staphylococcus aureus</i> VraS histidine kinase single point mutation on antibiotic resistanceSemantic proximity Read

A genome-wide atlas of antibiotic susceptibility targets and pathways to toleranceSemantic proximity Read

Multidrug Intrinsic Resistance Factors in Staphylococcus aureus Identified by Profiling Fitness within High-Diversity Transposon LibrariesSemantic proximity Read

Synergistic CRISPR-Cas Antimicrobials through Essential and Defensive Gene Cotargeting in <i>Staphylococcus aureus</i>Semantic proximity Read

Mobile-CRISPRi: Enabling Genetic Analysis of Diverse BacteriaSemantic proximity Read

Evolution under vancomycin selection drives divergent collateral sensitivity patterns in <i>Staphylococcus aureus</i>Semantic proximity Read