Article

LwaCas13a indiscriminately targets the human transcriptome

2025-05-16

Abstract excerpt

Background Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated protein 13a (Cas13a) has been described as a superior tool to short-interfering RNAs (siRNAs) for specific gene silencing. Cas13 targets RNAs through Watson-Crick binding of the target CRISPR RNA (crRNA) and activation of nuclease activity. In bacteria, once Leptotrichia wadei Cas13a (LwCas13a) has cut its specific target, th...

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Literature Corpus work: 48e10612-743d-583c-8992-f61f95204cab
DOI: 10.12688/wellcomeopenres.23656.1

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