Article

Deep learning and CRISPR-Cas13d ortholog discovery for optimized RNA targeting

2021-09-14

Abstract excerpt

Transcriptome engineering technologies that can effectively and precisely perturb mammalian RNAs are needed to accelerate biological discovery and RNA therapeutics. However, the broad utility of programmable CRISPR-Cas13 ribonucleases has been hampered by an incomplete understanding of the design rules governing guide RNA activity as well as cellular toxicity resulting from off-target or collateral RNA cleavage. H...

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Literature Corpus work: 4e4e2f30-37ef-5d57-97e0-d6f5f627c29b
DOI: 10.1101/2021.09.14.460134

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