Article

Allosteric activation of the SPRTN protease by ubiquitin maintains genome stability

2024-11-28

Abstract excerpt

The DNA-dependent protease SPRTN maintains genome stability by degrading toxic DNA-protein crosslinks (DPCs). To understand how SPRTN’s promiscuous protease activity is confined to the cleavage of crosslinked proteins, we reconstitute the repair of DPCs including their modification with SUMO and ubiquitin chains, using recombinant human proteins. We discover that DPC ubiquitylation strongly activates SPRTN indepen...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: aa4708a6-dc9d-5fa0-8bdb-ab39551b44f5
DOI: 10.1101/2024.11.26.625192

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Allosteric activation of the SPRTN protease by ubiquitin maintains genome stabilityDOI 10.1101/2024.11.26.625192Read

SPRTN is a mammalian DNA-binding metalloprotease that resolves DNA-protein crosslinks.Semantic proximity Read

Metalloprotease SPRTN/DVC1 Orchestrates Replication-Coupled DNA-Protein Crosslink Repair.Semantic proximity Read

Mechanisms and Regulation of DNA-Protein Crosslink Repair During DNA Replication by SPRTN ProteaseSemantic proximity Read

Structure of E3 ligase E6AP with a novel proteasome-binding site provided by substrate receptor hRpn10Semantic proximity Read

Structural basis of human TREX1 DNA degradation and autoimmune disease.Semantic proximity Read

FAM111A protects replication forks from protein obstacles via its trypsin-like domain.Semantic proximity Read

Structural basis of K63-ubiquitin chain formation by the Gordon-Holmes syndrome RBR E3 ubiquitin ligase RNF216.Semantic proximity Read

Tyrosyl-DNA phosphodiesterase 1 (TDP1) and SPRTN protease repair histone 3 and topoisomerase 1 DNA-protein crosslinks <i>in vivo</i>Semantic proximity Read

Mining the CRBN Target Space Redefines Rules for Molecular Glue-induced Neosubstrate RecognitionSemantic proximity Read

DNAJB8 oligomerization is mediated by an aromatic-rich motif that is dispensable for substrate activity.Semantic proximity Read

Nucleosomes specify co-factor access to p53Semantic proximity Read

Replication-associated formation and repair of human topoisomerase IIIα cleavage complexesSemantic proximity Read