Article

Robust and stable transcriptional repression in <i>Giardia</i> using CRISPRi

2018-06-28

Abstract excerpt

Giardia lamblia is a binucleate protistan parasite causing significant diarrheal disease worldwide. An inability to target Cas9 to both nuclei, combined with the lack of non-homologous end joining and markers for positive selection, has stalled the adaptation of CRISPR/Cas9-mediated genetic tools for this widespread parasite. CRISPR interference (CRISPRi) is a modification of the CRISPR/Cas9 system that directs ca...

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Literature Corpus work: a5e7b52e-b95a-5794-a2de-e19600fb83d0
DOI: 10.1101/358598

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