Article

Ribozyme-Mediated, Multiplex CRISPR Gene Editing and CRISPRi in <i>Plasmodium yoelii</i>

2018-11-29

Abstract excerpt

Functional characterization of genes in Plasmodium parasites often relies on genetic manipulations to disrupt or modify a gene-of-interest. However, these approaches are limited by the time required to generate transgenic parasites for P. falciparum and the availability of a single drug selectable marker for P. yoelii . In both cases, there remains a risk of disrupting native gene regulatory elements with the i...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 81d15333-d7a5-5585-86f3-dd27d15a8817
DOI: 10.1101/481416

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Ribozyme-Mediated, Multiplex CRISPR Gene Editing and CRISPRi in <i>Plasmodium yoelii</i>DOI 10.1101/481416Read

Ribozyme-mediated, multiplex CRISPR gene editing and CRISPR interference (CRISPRi) in rodent-infectious Plasmodium yoeliiSemantic proximity Read

Efficient Editing of Malaria Parasite Genome Using the CRISPR/Cas9 SystemSemantic proximity Read

CRISPR/Cas9 gene editing to make conditional mutants of the human malaria parasite <i>Plasmodium falciparum</i>Semantic proximity Read

A Conditional Cas9 System for Stage-Specific Gene Editing in <i>P. falciparum</i>Semantic proximity Read

CRISPR/Cas9 and genetic screens in malaria parasites: small genomes, big impactSemantic proximity Read

CRISPR-Cas13 in malaria parasite: Diagnosis and prospective gene function identificationSemantic proximity Read

Highly efficient CRISPR/Cas9 system in Plasmodium falciparum using Cas9-expressing parasites and a linear donor template.Semantic proximity Read

Generating conditional gene knockouts in Plasmodium – a toolkit to produce stable DiCre recombinase-expressing parasite lines using CRISPR/Cas9Semantic proximity Read

Cutting back malaria: CRISPR/Cas9 genome editing of PlasmodiumSemantic proximity Read

CRISPR/Cas advancements for genome editing, diagnosis, therapeutics, and vaccine development for Plasmodium parasites, and genetic engineering of Anopheles mosquito vectorSemantic proximity Read

CRISPR-mediated genome editing of Plasmodium falciparum malaria parasitesSemantic proximity Read

Constitutive expression of Cas9 and rapamycin-inducible Cre recombinase facilitates conditional genome editing in <i>Plasmodium berghei</i>Semantic proximity Read