Article

Use of CRISPR interference for efficient and rapid gene inactivation in <i>Fusobacterium nucleatum</i>

2023-09-20

Abstract excerpt

<h4>ABSTRACT</h4> Gene inactivation via creating in-frame deletion mutations in Fusobacterium nucleatum is time-consuming, and most fusobacterial strains are genetically intractable. Addressing these problems, we introduced a riboswitch-based inducible CRISPRi system. This system employs the nuclease-inactive Streptococcus pyogenes Cas9 protein (dCas9), specifically guided to the gene of interest by a constantl...

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Literature Corpus work: f85fb40f-f2a8-5775-8951-2916d082d451
DOI: 10.1101/2023.09.19.558491

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