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Inducible CRISPRi-based operon silencing and selective <i>in trans</i> gene complementation in <i>Borrelia burgdorferi</i>

2022-12-08

Abstract excerpt

<h4>ABSTRACT</h4> To accelerate genetic studies on the Lyme disease pathogen Borrelia burgdorferi , we developed an enhanced CRISPR interference (CRISPRi) approach for IPTG-inducible repression of specific B. burgdorferi genes. The entire system is encoded on a compact 11-kb shuttle vector plasmid that allows for inducible expression of both the sgRNA module and a non-toxic codon-optimized dCas9 protein. We val...

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Literature Corpus work
400c1075-a703-5e48-bb13-0274420a306b
DOI
10.1101/2022.12.05.519228
Open publication

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