Article

High-efficiency CRISPR gene editing in <i>C. elegans</i> using Cas9 integrated into the genome

2021-08-03

Abstract excerpt

Gene editing in C. elegans using plasmid-based CRISPR reagents requires microinjection of many animals to produce a single edit. Germline silencing of plasmid-borne Cas9 is a major cause of inefficient editing. Here, we present a set of C. elegans strains that constitutively express Cas9 in the germline from an integrated transgene. These strains markedly improve the success rate for plasmid-based CRISPR edits....

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: a163e754-bd2e-5128-81c9-76345deb7e9a
DOI: 10.1101/2021.08.03.454883

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

High-efficiency CRISPR gene editing in <i>C. elegans</i> using Cas9 integrated into the genomeDOI 10.1101/2021.08.03.454883Read

Modular safe-harbor transgene insertion (MosTI) for targeted single-copy and extrachromosomal array integration in <i>C. elegans</i>Semantic proximity Read

Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes.Semantic proximity Read

Precision genome editing using CRISPR-Cas9 and linear repair templates in C. elegans.Semantic proximity Read

Efficient marker-free recovery of custom genetic modifications with CRISPR/Cas9 in Caenorhabditis elegans.Semantic proximity Read

A Decade of CRISPR-Cas Gnome Editing in C. elegans.Semantic proximity Read

Transgene-free genome editing in Caenorhabditis elegans using CRISPR-Cas.Semantic proximity Read

Genome Editing in <i>Caenorhabditis briggsae</i> using the CRISPR/Cas9 SystemSemantic proximity Read

Modular gene tagging in <i>C. elegans</i>Semantic proximity Read

Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable Phenotype.Semantic proximity Read

FLInt: single shot safe harbor transgene integration via Fluorescent Landmark Interference.Semantic proximity Read

The application of somatic CRISPR-Cas9 to conditional genome editing in Caenorhabditis elegans.Semantic proximity Read

Single Nucleotide Substitutions Effectively Block Cas9 and Allow for Scarless Genome Editing in <i>Caenorhabditis elegans</i>Semantic proximity Read