Article

Modular gene tagging in <i>C. elegans</i>

2026-01-15

Abstract excerpt

Tagging a gene endogenously can identify when the gene is expressed and where the protein is localized. CRISPR is the primary tool for generating tags of endogenous genes, but it is error-prone and requires unique reagents for each gene and tag. Recombinases can insert DNA in an error-free and modular manner. Here, we tested eight recombinases for germline function in the nematode C. elegans , and introduce PhIT,...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 0ea5cb9b-ae70-598a-b45b-ee75f6553a67
DOI: 10.64898/2026.01.15.699741

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Modular gene tagging in <i>C. elegans</i>DOI 10.64898/2026.01.15.699741Read

Modular gene tagging in C. elegansSemantic proximity Read

High-efficiency CRISPR gene editing in <i>C. elegans</i> using Cas9 integrated into the genomeSemantic proximity Read

Modular safe-harbor transgene insertion (MosTI) for targeted single-copy and extrachromosomal array integration in <i>C. elegans</i>Semantic proximity Read

Rapid generation of <i>C. elegans</i> single copy transgenes combining RMCE and drug selectionSemantic proximity Read

SapTrap assembly of <i>C. elegans</i> MosSCI transgene vectorsSemantic proximity Read

Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes.Semantic proximity Read

CRIMP: a CRISPR/Cas9 insertional mutagenesis protocol and toolkit.Semantic proximity Read

Split-wrmScarlet and split-sfGFP: Tools for faster, easier fluorescent labeling of endogenous proteins in <i>Caenorhabditis elegans</i>Semantic proximity Read

A recombineering pipeline for functional genomics applied to Caenorhabditis elegans.Semantic proximity Read

Genome engineering by transgene-instructed gene conversion in C. elegans.Semantic proximity Read

Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable Phenotype.Semantic proximity Read

Expanding the <i>Caenorhabditis elegans</i> auxin-inducible degron system toolkit with internal expression and degradation controls and improved modular constructs for CRISPR/Cas9-mediated genome editingSemantic proximity Read