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Article

Transgene-free genome editing in Caenorhabditis elegans using CRISPR-Cas.

Genetics - 1 Nov 2013

Chiu Hui, Schwartz Hillel T, Antoshechkin Igor, Sternberg Paul W

Abstract excerpt

CRISPR-Cas is an efficient method for genome editing in organisms from bacteria to human cells. We describe a transgene-free method for CRISPR-Cas-mediated cleavage in nematodes, enabling RNA-homology-targeted deletions that cause loss of gene function; analysis of whole-genome sequencing indicates that the nuclease activity is highly specific.

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Identifiers

PMID: 23979577
DOI: 10.1534/genetics.113.155879
PMCID: PMC3813845

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Source receipt: pubmed, retrieved 7 Sept 2026.

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