Article
Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable Phenotype.
G3 (Bethesda, Md.) - 5 May 2017
El Mouridi Sonia, Lecroisey Claire, Tardy Philippe, Mercier Marine, Leclercq-Blondel Alice, Zariohi Nora, Boulin Thomas
Abstract excerpt
CRISPR/Cas9 genome engineering strategies allow the directed modification of the Caenorhabditis elegans genome to introduce point mutations, generate knock-out mutants, and insert coding sequences for epitope or fluorescent tags. Three practical aspects, however, complicate such experiments. First, the efficiency and specificity of single-guide RNAs (sgRNA) cannot be reliably predicted. Second, the detection of...
Topics
- Animals
- CRISPR-Cas Systems
- Caenorhabditis elegans
- Caenorhabditis elegans Proteins
- Collagen
- Gene Targeting
- Genome, Helminth
- Phenotype
- RNA, Guide, CRISPR-Cas Systems