Article
Efficient marker-free recovery of custom genetic modifications with CRISPR/Cas9 in Caenorhabditis elegans.
Genetics - 1 Nov 2014
Arribere Joshua A, Bell Ryan T, Fu Becky X H, Artiles Karen L, Hartman Phil S, Fire Andrew Z
Abstract excerpt
Facilitated by recent advances using CRISPR/Cas9, genome editing technologies now permit custom genetic modifications in a wide variety of organisms. Ideally, modified animals could be both efficiently made and easily identified with minimal initial screening and without introducing exogenous sequence at the locus of interest or marker mutations elsewhere. To this end, we describe a coconversion strategy, using...
Topics
- Alleles
- Animals
- Base Sequence
- CRISPR-Cas Systems
- Caenorhabditis elegans
- Chromosomes
- Crosses, Genetic
- DNA End-Joining Repair
- Female
- Gene Conversion
- Genetic Loci