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Article

Precision genome editing using CRISPR-Cas9 and linear repair templates in C. elegans.

Methods (San Diego, Calif.) - 15 May 2017

Paix Alexandre, Folkmann Andrew, Seydoux Geraldine

Abstract excerpt

The ability to introduce targeted edits in the genome of model organisms is revolutionizing the field of genetics. State-of-the-art methods for precision genome editing use RNA-guided endonucleases to create double-strand breaks (DSBs) and DNA templates containing the edits to repair the DSBs. Following this strategy, we have developed a protocol to create precise edits in the C. elegans genome. The protocol...

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Identifiers

PMID: 28392263
DOI: 10.1016/j.ymeth.2017.03.023
PMCID: PMC6788293

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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