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Single Nucleotide Substitutions Effectively Block Cas9 and Allow for Scarless Genome Editing in <i>Caenorhabditis elegans</i>

2021-09-14

Abstract excerpt

In Caenorhabditis elegans , germline injection of Cas9 complexes is reliably used to achieve genome editing through homology-directed repair of Cas9-generated DNA breaks. To prevent Cas9 from targeting repaired DNA, additional blocking mutations are often incorporated into homologous repair templates. Cas9 can be blocked either by mutating the PAM sequence that is essential for Cas9 activity or by mutating the gu...

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Identifiers and source

Literature Corpus work
47f8ad78-5b87-5806-a64b-aaae5099e986
DOI
10.1101/2021.09.14.460298
Open publication

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Single Nucleotide Substitutions Effectively Block Cas9 and Allow for Scarless Genome Editing in <i>Caenorhabditis elegans</i>DOI 10.1101/2021.09.14.460298
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