Article

Genome-scale perturb-seq in primary human CD4+ T cells maps context-specific regulators of T cell programs and human immune traits

2025-12-24

Abstract excerpt

Gene regulatory networks encode the fundamental logic of cellular functions, but systematic network mapping remains challenging, especially in cell states relevant to human biology and disease. Here, we perturbed all expressed genes across 22 million primary human CD4+ T cells from four donors and developed a probe-based perturb-seq platform to measure the transcriptome effects in cells at rest and after stimulati...

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Literature Corpus work: a088b0a8-2845-57b1-921e-b7e1da3ae086
DOI: 10.64898/2025.12.23.696273

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