Article

Instantaneous visual genotyping and facile site-specific transgenesis via CRISPR-Cas9 and phiC31 integrase

2022-11-01

Abstract excerpt

The zebrafish Danio rerio has become a popular model in functional genomics and genetic disease studies. However, when a zebrafish mutant line must be propagated as heterozygotes due to homozygous lethality, using standard genotyping methods to identify a population of homozygous mutant embryos is time-consuming and sometimes impractical due to downstream applications such as large-scale chemical screens. Here, w...

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Literature Corpus work: 9cf2e967-ff2c-57e1-a6be-ccad846e5e43
DOI: 10.1101/2022.10.30.514454

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