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Article

Precise and efficient genome editing in zebrafish using the CRISPR/Cas9 system.

Development (Cambridge, England) - 1 Dec 2014

Irion Uwe, Krauss Jana, Nüsslein-Volhard Christiane

Abstract excerpt

The introduction of engineered site-specific DNA endonucleases has brought precise genome editing in many model organisms and human cells into the realm of possibility. In zebrafish, loss-of-function alleles have been successfully produced; however, germ line transmission of functional targeted knock-ins of protein tags or of SNP exchanges have not been reported. Here we show by phenotypic rescue that the...

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Identifiers

PMID: 25411213
DOI: 10.1242/dev.115584
PMCID: PMC4299274

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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