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Article

Improved selection of zebrafish CRISPR editing by early next-generation sequencing based genotyping.

Scientific reports - 27 Jan 2023

Sieliwonczyk Ewa, Vandendriessche Bert, Claes Charlotte, Mayeur Evy, Alaerts Maaike, Holmgren Philip, Canter Cremers Tycho, Snyders Dirk, Loeys Bart, Schepers Dorien

Abstract excerpt

Despite numerous prior attempts to improve knock-in (KI) efficiency, the introduction of precise base pair substitutions by the CRISPR-Cas9 technique in zebrafish remains challenging. In our efforts to generate KI zebrafish models of human CACNA1C mutations, we have tested the effect of several CRISPR determinants on KI efficiency across two sites in a single gene and developed a novel method for early selection...

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Identifiers

PMID: 36707549
DOI: 10.1038/s41598-023-27503-9
PMCID: PMC9883431

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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