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Article

CRIMP: a CRISPR/Cas9 insertional mutagenesis protocol and toolkit.

Nature communications - 12 Jun 2024

Miles Lee B, Calcinotto Vanessa, Oveissi Sara, Serrano Rita J, Sonntag Carmen, Mulia Orlen, Lee Clara, Bryson-Richardson Robert J

Abstract excerpt

Site-directed insertion is a powerful approach for generating mutant alleles, but low efficiency and the need for customisation for each target has limited its application. To overcome this, we developed a highly efficient targeted insertional mutagenesis system, CRIMP, and an associated plasmid toolkit, CRIMPkit, that disrupts native gene expression by inducing complete transcriptional termination, generating...

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Identifiers

PMID: 38866742
DOI: 10.1038/s41467-024-49341-7
PMCID: PMC11169554

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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