Article
Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system.
Mammalian genome : official journal of the International Mammalian Genome Society - 1 Aug 2014
Mizuno Seiya, Dinh Tra Thi Huong, Kato Kanako, Mizuno-Iijima Saori, Tanimoto Yoko, Daitoku Yoko, Hoshino Yoshikazu, Ikawa Masahito, Takahashi Satoru, Sugiyama Fumihiro, Yagami Ken-ichi
Abstract excerpt
Single nucleotide mutations (SNMs) are associated with a variety of human diseases. The CRISPR/Cas9 genome-editing system is expected to be useful as a genetic modification method for production of SNM-induced mice. To investigate whether SNM-induced mice can be generated by zygote microinjection of CRISPR/Cas9 vector and single-stranded DNA (ssDNA) donor, we attempted to produce albino C57BL/6J mice carrying the...
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