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Article

Instantaneous visual genotyping and facile site-specific transgenesis via CRISPR-Cas9 and phiC31 integrase.

Biology open - 15 Sept 2024

Ma Junyan, Zhang Weiting, Rahimialiabadi Simin, Ganesh Nikkitha Umesh, Sun Zhengwang, Parvez Saba, Peterson Randall T, Yeh Jing-Ruey Joanna

Abstract excerpt

Here, we introduce 'TICIT', targeted integration by CRISPR-Cas9 and integrase technologies, which utilizes the site-specific DNA recombinase - phiC31 integrase - to insert large DNA fragments into CRISPR-Cas9 target loci. This technique, which relies on first knocking in a 39-basepair phiC31 landing site via CRISPR-Cas9, enables researchers to repeatedly perform site-specific transgenesis at the exact genomic...

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Identifiers

PMID: 39225039
DOI: 10.1242/bio.061666
PMCID: PMC11391820

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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